Usually when you do a ISCV reset you click it and it backs the Throttle Stepper Motor out fully so you can set the TPS to .6 volts + or - .02 volts then you click it again and it moves the stepper motor in and you check to see if the movement made the the throttle position change by .12 volts thus your new reading would be .72 volts again + or - .02 volts.
I would say the first thign you should do is verify you linked up with the ECU. This is pretty easy just do a fan test, a throttle stepper motor test, or a tach test works great also. in each case your fan should cycle on, or you will hear the throttle stepper motor move, and lastly you would see the tach sweap. These are good ways to insure the lap top is hooked to the ECU. then we could go from there.
The nut your adjusting is on the end of the idle stepper motor when you do this the OEM way or when initally setting up the stepper motor you would check the gap between the stepper motor roller and the throttle cam.
this can now be done electronically with the new tune softwares.
below is a picture of the nut I was referring to.
below is the cam and roller you would check with a feeler gauge to see if you have the right gap. But now days this gap is measured electronically by registering the .12 volt movement of the stepper motor in relation to the TPS sensor movement.
TPS Close =.60v then the ecu moves it + .12v for a total od .72v final reading during the test. (+ or - .02 deviation for a total of .04 votage tolerance)
Sorry about the fancy pointer I borrowed it from my grand daughter
Hope this helps some, as I pointed out see if you are connected by performing some of the simple test. Then see if the bugger will copy a map. I believe you should also have the map that you are going to use/modify/or install opened up when performing simple diagnotstics. In tune boy it would not matter because they are two seperate programs. one being tune boy dash and the other being tune edit.