If you use a custom tune whether you downloaded it or made it yourself, you should probably turn the O2 sensor off....otherwise your custom maps will eventually be overwritten by the 02 sensors data...if the ECU is getting o2 data it will write maps that go with its basic programming....rideable but as green as the technology it possesses. If your custom tune is working for you, you don't want it changed by the o2 sensor...and if the o2 sensor is switched off in ecu it will default to your custom maps.
Also the previous poster has a point...you can use a sensor eliminator and leave 02 turned on...in that case the ecu gets a dummy signal that everything is fine and it will use your custom map as written, confident that it is perfect as is.